カワムラ　ノブユキ   Nobuyuki KAWAMURA   川村　暢幸    所属   新潟薬科大学  薬学部 薬学科    職種   准教授
発表年月日	2014/10/21
発表テーマ	Upregulation of Vacuolar-type ATPase G1 Subunit by a Genetic Loss of Subunit G2 in Neuron
会議名	The 15th IUBMB-24th FAOBMB-TSBMB Conference Biochemistry and Molecular Biology in Transition:from Basic to Translational
学会区分	国際学会
発表形式	ポスター
開催地名	The Academia Sinica (Taipei, Taiwan)
発表者・共同発表者	Nobuyuki Kawamura,Ge-Hong Sun-Wada,Yoh Wada
概要	Vacuolar type ATPase (V-ATPase) is a primary proton pump with versatile function in various tissues. In nerve cells, V-ATPase is required for accumulation of neurotransmitters into secretory vesicles and subsequent release at synapse. Neurons express a specific isoform (G2) for the G subunit of V-ATPase constituting the catalytic sector of enzyme complex. We generated a mouse lacking the functional G2 by gene targeting. G2 null animals exhibited none of apparent disorders in their architecture and behavior. In brain of G2-null mouse, G1 subunit isoform, which is ubiquitously expressed in neuronal and non-neuronal tissues, is more abundantly accumulated comparing to the wild-type animals. This upregulation of the G1 was not accompanied with the increase of mRNA. These results indicated that the function of neuron specific G2 isoform is compensated by an increase of the G1 isoform, whose accumulation levels were controlled by post-transcriptional regulatory mechanism.