カワムラ　ノブユキ   Nobuyuki KAWAMURA   川村　暢幸    所属   新潟薬科大学  薬学部 薬学科    職種   准教授
言語種別	英語
発行・発表の年月	2022/08
形態種別	論文
査読	査読あり
標題	Importance of two-dimensional cation clusters induced by protein folding in intrinsic intracellular membrane permeability.
執筆形態	共著
掲載誌名	RSC chemical biology
掲載区分	国外
巻・号・頁	3(8),pp.1076-1084
著者・共著者	Negi Shigeru, Hamori Mami, Kawahara-Nakagawa Yuka, Imanishi Miki, Kurehara Miku, Kitada Chieri, Kawahito Yuri, Kishi Kanae, Manabe Takayuki, Kawamura Nobuyuki, Kitagishi Hiroaki, Mashimo Masato, Shibata Nobuhito, Sugiura Yukio
概要	We investigated the cell penetration of Sp1 zinc finger proteins (Sp1 ZF) and the mechanism via which the total cationic charge and distribution of cationic residues on the protein surface affect intracellular trafficking. Sp1 ZFs showed intrinsic cell membrane permeability. The intracellular transfer of Sp1 ZFs other than 1F3 was dependent on the total cationic charge. Investigation of the effect of cationic residue distribution on intracellular membrane permeability revealed that the cellular uptake of unfolded Zn2+-non-coordinating Ala mutants was lower than that of the wild type. Therefore, the total cationic charge and distribution of cationic residues on the protein played crucial roles in intracellular translocation. Mutational studies revealed that the two-dimensional cation cluster on the protein surface significantly improved their cellular uptake. This study will contribute to the design of artificial cargoes that can efficiently transport target substances into cells.
DOI	10.1039/d2cb00098a
ISSN	2633-0679
PMID	35975000