ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	1999/04
形態種別	論文
査読	査読あり
標題	Conversion of a bacterial warm sensor to a cold sensor by methylation of a single residue in the presence of an attractant.
執筆形態	共著
掲載誌名	Molecular microbiology
掲載区分	国内
巻・号・頁	32(2),pp.357-65
著者・共著者	◎Nishiyama S, Umemura T, Nara T, Homma M, Kawagishi I
概要	The aspartate chemoreceptor (Tar) of Escherichia coli also serves as a thermosensor. Its thermosensing properties are controlled by reversible methylation of the cytoplasmic signalling/adaptation domain of the protein. The unmethylated and the fully methylated (aspartate-bound) receptors sense, as attractant stimuli, increases (warm sensor) and decreases (cold sensor) in temperature respectively. In this study, we replaced the four methyl-accepting glutamyl residues with non-methylatable aspartyl residues in all possible combinations. Without any modification, all of the mutant Tar proteins functioned as warm sensors. Almost all of the methylated mutant receptors were cold sensors in the presence of aspartate. Thus, binding of aspartate to Tar and methylation of its single glutamyl residue can invert its temperature-dependent signalling properties.
ISSN	0950-382X
PMID	10231491