オオツボ　ケンイチ   OHTSUBO KEN-ICHI   大坪　研一    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   特任教授
言語種別	英語
発行・発表の年月	2020/07
形態種別	論文
査読	査読あり
標題	Development of a formula for estimating the amylose content of starch using japonica milled rice flours based on the iodine absorption curve
執筆形態	共著
掲載誌名	Bioscience, Biotechnology, and Biochemistry
掲載区分	国内
出版社・発行元	日本農芸化学会
巻・号・頁	84(doi.org/10.1080/09168451.2020.1794786)
著者・共著者	Sumiko Nakamura, Hiroki Yamaguchi, Yuu Benitani, Ken'ichi Ohtsubo
概要	The apparent amylose content (AAC) is usually measured by an iodine colorimetric method using the starch as a sample. Nevertheless, it is time-consuming and labor-intensive to prepare rice starch. Therefore, we compared two methods, starch method and milled rice flour method. The former AACs were higher than the latter and correlated with pasting properties, physical properties and sensory scores better than the latter. Therefore, we developed a novel formula for estimating the AACs of starches from japonica rice cultivars using milled rice flours instead of using rice starch. The correlation coefficients of the new formula were 0.89 for calibration and 0.94 for validation, which showed that this equation can be applied to unknown japonica rice samples, and will lead to easy, rapid, and accurate iodine method to determine rice AACs using milled rice flours instead of starch.