ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2007/06
形態種別	論文
査読	査読あり
標題	Involvement of minor components associated with the FimA fimbriae of Porphyromonas gingivalis in adhesive functions.
執筆形態	共著
掲載誌名	Microbiology (Reading, England)
掲載区分	国外
巻・号・頁	153(Pt 6),pp.1916-25
著者・共著者	◎Nishiyama So-ichiro, Murakami Yukitaka, Nagata Hideki, Shizukuishi Satoshi, Kawagishi Ikuro, Yoshimura Fuminobu
概要	The FimA fimbriae of Porphyromonas gingivalis, the causative agent of periodontitis, have been implicated in various aspects of pathogenicityn. In this study, the four open reading frames (ORF1-4) downstream of the fimbrilin gene (fimA) in strain ATCC 33277 were examined. ORF2-4 were demonstrated to encode minor components of the fimbriae and were therefore renamed fimC, fimD and fimE, respectively. The fimE mutant produced FimC and FimD, but fimbriae purified from it contained neither protein, suggesting that FimE is required for the assembly of FimC and FimD onto the FimA fimbriae. The fimC, fimD and fimE mutants lost autoaggregation abilities. Fimbriae purified from these three mutants showed attenuated binding activities to fibronectin and type I collagen. These results suggest that FimE, as well as FimC and FimD, play critical roles in the adhesive activities of the mature FimA fimbriae in P. gingivalis.
DOI	10.1099/mic.0.2006/005561-0
ISSN	1350-0872
PMID	17526848