ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	1999/03
形態種別	論文
査読	査読あり
標題	Inversion of thermosensing property of the bacterial receptor Tar by mutations in the second transmembrane region.
執筆形態	共著
掲載誌名	Journal of molecular biology
掲載区分	国外
巻・号・頁	286(5),pp.1275-84
著者・共著者	◎Nishiyama S, Maruyama I N, Homma M, Kawagishi I
概要	The aspartate chemoreceptor Tar of Escherichia coli serves as a warm sensor that produces attractant and repellent signals upon increases and decreases in temperature, respectively. However, increased levels of methylation of the cytoplasmic domain of Tar resulting from aspartate binding convert Tar to a cold sensor with the opposite signaling behavior. As an initial step toward understanding the role of the second transmembrane region (TM2) in thermosensing, we have examined the thermosensing properties of 43 mutant Tar receptors with randomized TM2 sequences (residues 190-210). Among them, we identified one mutant receptor (Tar-I2) that functioned as a cold sensor in the absence of aspartate. This is the first example of attractant-independent inversion of thermosensing in Tar. Thus, displacements of TM2 seem to influence the thermosensing function of Tar.
ISSN	0022-2836
PMID	10064695