ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2019/05
形態種別	論文
査読	査読あり
標題	Calcium ion modulates amino acid sensing of the chemoreceptor Mlp24 of Vibrio cholerae
執筆形態	共著
掲載誌名	Journal of Bacteriology
掲載区分	国外
出版社・発行元	American Society for Microbiology
巻・号・頁	201,pp.e00779-18
著者・共著者	◎Takahashi Yohei, Nishiyama So-ichiro, Sumita Kazumasa, Kawagishi Ikuro, and Imada Katsumi
概要	Mlp24 and Mlp37 are homologous chemoreceptors of Vibrio cholerae that bind various amino acids. Although most of the residues involved in ligand interaction are conserved, these chemoreceptors show different affinity for the same ligand and play different cellular roles. A series of ligand complex structures of the periplasmic region of Mlp24 (Mlp24p) and following ITC anaylysis revealed that Ca2+ binds to the loop of Mlp24p and modulates the ligand binding affinity of Mlp24p. Moreover, Ca2+ changes the chemotactic behaviors mediated by Mlp24. We propose that Ca2+ acts as a co-signal that modulates the affinity of Mlp24 for the primary signal, thereby changes the chemotactic behavior of V. cholerae.