ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2010/09
形態種別	論文
査読	査読あり
標題	FimB regulates FimA fimbriation in Porphyromonas gingivalis.
執筆形態	共著
掲載誌名	Journal of dental research
掲載区分	国外
巻・号・頁	89(9),pp.903-8
著者・共著者	◎Nagano K, Hasegawa Y, Murakami Y, Nishiyama S, Yoshimura F
概要	The periodontitis-associated pathogen Porphyromonas gingivalis colonizes and forms a biofilm in gingival crevices through fimbriae. It is known that the often-used strains ATCC 33277 and 381 produce long FimA fimbriae. We found a possible nonsense mutation within fimB, immediately downstream from fimA, coding a major subunit of FimA fimbriae of the strains. Electron micrographs revealed that a FimB-restored strain had short and dense, "toothbrush"-like, FimA fimbriae. FimB restoration increased production of FimA and its accessory proteins. Thus, FimB regulates the length and expression of FimA fimbriae. Additionally, FimB restoration significantly reduced the release of FimA fimbriae from the cell surface, suggesting that FimB functions as an anchor of the fimbriae. The restoration enhanced adherent activity as well.
DOI	10.1177/0022034510370089
ISSN	1544-0591
PMID	20530728