ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2003
形態種別	論文
査読	査読あり
標題	A novel type of two-component regulatory system affecting gingipains in Porphyromonas gingivalis.
執筆形態	共著
掲載誌名	Microbiology and immunology
掲載区分	国内
巻・号・頁	47(11),pp.849-58
著者・共著者	◎Hasegawa Yoshiaki, Nishiyama So-ichiro, Nishikawa Kiyoshi, Kadowaki Tomoko, Yamamoto Kenji, Noguchi Toshihide, Yoshimura Fuminobu
概要	We surveyed the Porphyromonas gingivalis genome database for homologues of FimS, the first two-component sensor histidine kinase, which could possibly control virulence factors. Including fimS, we found six putative sensor kinase genes in the genome. The gene encoding one of the homologues was cloned, sequenced, and analyzed using its mutants. The mutants formed nonpigmented colonies, indicating that they might be defective in proteinase production. Proteinase activities of the mutants were almost half those of the parent strain. Interestingly, most of the gingipain activities were detected in the culture supernatant, not in cells, of the mutants. These results strongly suggest that this newly-discovered two-component sensor kinase is involved in maturation and proper localization of its proteases. The gene was designated gppX, which represents regulation (X) of gingipains and black pigmentation in P. gingivalis.
ISSN	0385-5600
PMID	14638996