ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2015/03
形態種別	論文
査読	査読あり
標題	Hypoxia-induced localization of chemotaxis-related signaling proteins in Vibrio cholerae.
執筆形態	共著
掲載誌名	Molecular microbiology
掲載区分	国外
巻・号・頁	95(5),pp.780-90
著者・共著者	◎Hiremath Geetha, Hyakutake Akihiro, Yamamoto Kentaro, Ebisawa Tatsuaki, Nakamura Tomoyuki, Nishiyama So-ichiro, Homma Michio, Kawagishi Ikuro
概要	Vibrio cholerae has three sets of chemotaxis-related signaling proteins, of which only System II has been shown to be involved in chemotaxis. Here, we examined localization of green fluorescent protein (GFP)-fused components of System I. The histidine kinase (CheA1) and the adaptor (CheW0) of System I localized to polar and lateral membrane regions with standing incubation (microaerobic conditions), but their localization was lost after shaking (aerobic conditions). A transmembrane receptor of System I also showed polar and lateral localization with standing incubation. Nitrogen gas, sodium azide or carbonylcyanide m-chlorophenylhydrazone induced localization of CheA1-GFP even with shaking incubation, suggesting that the localization is controlled in response to changes in energy metabolism. These results suggest that System I components are assembled into the supramolecular signaling complex in response to reduced cellular energy states.
DOI	10.1111/mmi.12887
ISSN	1365-2958
PMID	25420689