ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2012/09
形態種別	論文
査読	査読あり
標題	Mlp24 (McpX) of Vibrio cholerae implicated in pathogenicity functions as a chemoreceptor for multiple amino acids.
執筆形態	共著
掲載誌名	Infection and immunity
掲載区分	国外
巻・号・頁	80(9),pp.3170-8
著者・共著者	◎Nishiyama So-ichiro, Suzuki Daisuke, Itoh Yasuaki, Suzuki Kazuho, Tajima Hirotaka, Hyakutake Akihiro, Homma Michio, Butler-Wu Susan M, Camilli Andrew, Kawagishi Ikuro
概要	The chemotaxis of Vibrio cholerae, the causative agent of cholera, has been implicated in pathogenicity. The bacterium has more than 40 genes for MLPs. In this study, we found that glycine and at least 18 L-amino acids serve as attractants to the classical biotype strain O395N1. Among the MLPs, Mlp24 is required for the production of cholera toxin upon mouse infection. mlp24 deletion strains showed defects in taxis toward several amino acids. Serine, arginine, asparagine, and proline were shown to bind directly to the periplasmic fragment of Mlp24. These results suggest that Mlp24 is a chemoreceptor for multiple amino acids, including serine, arginine, and asparagine, which were previously shown to stimulate the expression of several virulence factors, implying that taxis toward a set of amino acids plays critical roles in pathogenicity of V. cholerae.
DOI	10.1128/IAI.00039-12
ISSN	1098-5522
PMID	22753378