オオタケサトウ　マルセロ   Marcello Otake Sato    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2025/10
形態種別	論文（従来区分）
査読	査読あり
標題	Soil-based environmental DNA enables detection of Oncomelania hupensis quadrasi and Schistosoma japonicum microhabitats for schistosomiasis japonica surveillance and control in the Philippines.
執筆形態	共著（従来区分）
掲載誌名	Infectious diseases of poverty
掲載区分	国外
出版社・発行元	Springer Nature
巻・号・頁	14(1),pp.110
担当区分	責任著者
国際共著	国際共著
著者・共著者	Joseph E Valencia, Marcello Otake Sato, Mark June Revolteado, Phoyphaylinh Prasayasith, Mario Jiz, Eleonor A Cervantes, Ralph N Aniceto, Marianette Inobaya, Pengfei Cai, Darren J Gray, Catherine A Gordon, Lydia R Leonardo, Yasuhito Sako, Megumi Sato
概要	BACKGROUND:Schistosomiasis japonica, caused by Schistosoma japonicum, remains a significant public health concern in the Philippines, where 12.4 million people are at risk due to persistent transmission in endemic regions. The distribution of schistosomiasis is closely linked to the distribution of its snail intermediate host. This study aims to assess the potential of using soil samples to detect the environmental presence of Oncomelania hupensis quadrasi and Schistosoma japonicum.METHODS:This cross-sectional observational study utilized a soil-based environmental DNA (eDNA) detection system for simultaneous detection of O. h. quadrasi, and S. japonicum mitochondrially encoded cytochrome c oxidase subunit 1 gene in multiplex quantitative real-time PCR and digital PCR platforms. A two-phase sample collection and testing were carried out in December 2023 (Phase 1) and March 2024 (Phase 2) across 30 selected sampling sites in Ekiran village, Leyte, Philippines. Wilcoxon two-sample/Mann-Whitney U test was used to determine whether there was a significant difference in eDNA presence and edaphic factors, while percent agreement was used to assess the concordance among methods.RESULTS:This study reveals that S. japonicum eDNA can be detected in soil samples and confirms the strong applicability of soil-based eDNA for detecting O. h. quadrasi snails and even in sites without visible snail presence. Furthermore, it demonstrates the superiority of soil-eDNA system compared to classical malacological surveys in detecting O. h. quadrasi and S. japonicum microhabitats: in Phase 1, eDNA detected O. h. quadrasi and S. japonicum in 50% (3/6) and 66.67% (4/6) of sites, respectively, while malacological surveys detected them in only 50% and 16.67% (1/6) of sites. In Phase 2, eDNA detected O. h. quadrasi in 20% (6/30) of sites compared to only 10% (3/30) by malacological survey, and S. japonicum was detected only by eDNA in 10% (3/30) of sites. Among the measured soil parameters, only pH showed a statistically significant difference between eDNA-positive and eDNA-negative sites (P = 0.04).CONCLUSIONS:Soil-based eDNA sensitively detected O. h. quadrasi and S. japonicum, enabling scalable, non-invasive transmission site identification and outperforming traditional surveys without visible snails. Its ability to detect S. japonicum highlights its value for comprehensive schistosomiasis monitoring.
DOI	10.1186/s40249-025-01374-w
ISSN	2049-9957
PMID	41168821