|
トウ ヒデヒロ
藤 英博 所属 新潟薬科大学 応用生命科学部 学部付 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2024/09 |
| 形態種別 | 論文 |
| 査読 | 査読あり |
| 標題 | Mitochondrial phylogeny and distribution of cytoplasmic male sterility-associated genes in Beta vulgaris |
| 執筆形態 | 共著 |
| 掲載誌名 | PLOS ONE |
| 掲載区分 | 国外 |
| 出版社・発行元 | Public Library of Science (PLoS) |
| 巻・号・頁 | 19(9),pp.e0308551 |
| 著者・共著者 | Keishi Kubota,Mion Oishi,Eigo Taniguchi,Akiho Akazawa,Katsunori Matsui,Kazuyoshi Kitazaki,Atsushi Toyoda,Hidehiro Toh,Hiroaki Matsuhira,Yosuke Kuroda,Tomohiko Kubo |
| 概要 | Cytoplasmic male sterility (CMS) is a mitochondrial-encoded trait that confers reproductive defects in males but not in females or any vegetative function. Why CMS is so often found in plants should be investigated from the viewpoint of mitochondrial phylogeny. Beta vulgaris, including the wild subspecies maritima and cultivated subspecies vulgaris (e.g., sugar beet), is known to be mitochondrially polymorphic, from which multiple CMS mitochondria have been found, but their evolutionary relationship has been obscure. We first refined the B. vulgaris reference mitochondrial genome to conduct a more accurate phylogenetic study. We identified mitochondrial single-nucleotide polymorphic sites from 600 B. vulgaris accessions. Principal component analysis, hierarchical clustering analysis, and creation of a phylogenetic tree consistently suggested that B. vulgaris mitochondria can be classified into several groups whose geographical distribution tends to be biased toward either the Atlantic or Mediterranean coasts. We examined the distribution of CMS-associated mitochondrial genes from Owen, E- and G-type CMS mitochondria. About one-third of cultivated beets had Owen-type CMS, which reflects the prevalence of using Owen-type CMS in hybrid breeding. Occurrence frequencies for each of the three CMS genes in wild beet were less than 4%. CMS genes were tightly associated with specific mitochondrial groups that are phylogenetically distinct, suggesting their independent origin. However, homologous sequences of the Owen type CMS gene occurred in several different mitochondrial groups, for which an intricate explanation is necessary. Whereas the origin of cultivated beet had been presumed to be Greece, we found an absence of Owen-type mitochondria in Greek accessions. |
| DOI | 10.1371/journal.pone.0308551 |
| ISSN | 1932-6203 |
| PermalinkURL | https://dx.plos.org/10.1371/journal.pone.0308551 |