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オオタケサトウ マルセロ
Marcello Otake Sato 所属 新潟薬科大学 医療技術学部 臨床検査学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/12 |
| 形態種別 | 論文 |
| 査読 | 査読あり |
| 標題 | Evaluation of Purified Taenia solium Glycoproteins and Recombinant Antigens in the Serologic Detection of Human and Swine Cysticercosis |
| 執筆形態 | 共著 |
| 掲載誌名 | Journal of Infectious Diseases |
| 掲載区分 | 国外 |
| 巻・号・頁 | 194(12),pp.1783-1790 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | ◎Sato, Marcello Otake; Sako, Yasuhito; Nakao, Minoru; Yamasaki, Hiroshi; Nakaya, Kazuhiro; Ito, Akira |
| 概要 | Cysticercosis caused by infection with embryonated eggs of Taenia solium is an important cause of neurological disease worldwide. On the basis of mitochondrial DNA analysis, T. solium is divided into 2 (African/American and Asian) genotypes. Glycoproteins (GPs) in cyst fluid purified from the 2 genotypes of T. solium were characterized and compared with the recombinant chimeric T. solium–Ag1V1/Ag2 protein (Rec‐Ag1V1/Ag2) as serodiagnostic antigens. Immunoblot analysis revealed that banding patterns of GPs differed between the 2 genotypes because of posttranslation modification, especially glycosylation. The comparison of native GPs with Rec‐Ag1V1/Ag2 by enzyme‐linked immunosorbent assay demonstrated that there was no statistical difference in sensitivity. In addition, the conservation of the genes encoding Ag1V1 and Ag2 in T. solium worldwide was verified. These results indicate that Rec‐Ag1V1/Ag2 has great potential for usefulness in serodiagnosis as an alternative to native antigens. |
| DOI | https://doi.org/10.1086/509262 |
| ISSN | 0022-1899/1537-6613 |