|
ワタナベ ケイスケ
渡辺 啓介 所属 新潟薬科大学 医療技術学部 臨床検査学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2005/12 |
| 形態種別 | 論文 |
| 査読 | 査読あり |
| 標題 | Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland |
| 執筆形態 | 共著 |
| 掲載誌名 | CELL AND TISSUE RESEARCH |
| 掲載区分 | 国外 |
| 出版社・発行元 | SPRINGER |
| 巻・号・頁 | 322(3),pp.455-462 |
| 著者・共著者 | Y Nagai,T Nochi,K Watanabe,K Watanabe,H Aso,H Kitazawa,M Matsuzaki,S Ohwada,T Yamaguchi |
| 概要 | A pro-inflammatory cytokine, interleukin 18 (IL-18), induces intracellular expression of IL-1 and the release of IL-6. IL-1 and IL-6 has been detected in anterior pituitary cells, suggesting that IL-18 is produced in anterior pituitary cells and may serve to aid immuno-endocrine regulation. In the present study, we addressed this hypothesis by investigating the intracellular localization of IL-18 and its receptor in bovine anterior pituitary gland. IL-18 mRNA and its protein were detected in the anterior pituitary gland by RT-PCR and Western blotting. In situ hybridization showed that IL-18 mRNA was expressed in the anterior pituitary cells. Immunohistochemistry of IL-18 and specific hormones revealed the presence of IL-18 in somatotrophs. Furthermore, the expression of GH mRNA in IL-18 immunoreactive cells was confirmed by immuno-laser microdissection. These results first demonstrated that somatotrophs produced IL-18. Subsequently, the distribution of the IL-18 receptor alpha (IL-18R alpha) was investigated in order to understand IL-18 signaling among the anterior pituitary cells. Bovine IL-18R alpha cDNA was partially sequenced and detected in the anterior pituitary gland by RT-PCR. Immunohistochemistry of IL-18R alpha, IL-18 and GH showed that IL-18R alpha was co-localized in IL-18 immunoreactive cells or somatotrophs. These data suggest that IL-18 acts on somatotrophs as an immuno-endocrine mediator through the autocrine pathway. |
| DOI | 10.1007/s00441-005-0016-0 |
| ISSN | 0302-766X |
| PMID | 16001266 |