ノズミ　モトヒロ   野住　素広    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2008/06
形態種別	論文 - 研究論文（学術雑誌）
査読	査読あり
標題	A novel method for RNA interference in neurons using enhanced green fluorescent protein (EGFP)-transgenic rats.
執筆形態	その他 - 指定なし
掲載誌名	Neuroscience research
掲載区分	国外
巻・号・頁	61(2),pp.219-24
著者・共著者	Jia Lu,Motohiro Nozumi,Hiroshi Fujii,Michihiro Igarashi
概要	RNA interference (RNAi) is the simplest way of examining gene function by inhibiting expression. However, due to the low rate of introducing short interfering RNA (siRNA) into neurons, it is difficult to discriminate into which neurons that have been successfully introduced. Here, we used neurons from transgenic rats expressing enhanced green fluorescent protein (EGFP), and we simultaneously applied small interfering RNAs (siRNAs) against EGFP and a targeted gene to the EGFP-expressing neurons. EGFP fluorescence and immunoreactivity of the protein were then assessed by immunofluorescence microscopy. Quantitative analysis of the immunofluorescence confirmed that loss of EGFP closely correlates with loss of the target protein. These results indicate that this method can be used in a wider range of the neuroscientific research, especially in genome-wide studies.
DOI	10.1016/j.neures.2008.02.008
PMID	18423674