ノズミ　モトヒロ   野住　素広    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2009/10/06
形態種別	論文 - 研究論文（学術雑誌）
査読	査読あり
標題	Identification of functional marker proteins in the mammalian growth cone.
執筆形態	その他 - 指定なし
掲載誌名	Proceedings of the National Academy of Sciences of the United States of America
掲載区分	国外
巻・号・頁	106(40),pp.17211-6
担当区分	筆頭著者
著者・共著者	Motohiro Nozumi,Tetsuya Togano,Kazuko Takahashi-Niki,Jia Lu,Atsuko Honda,Masato Taoka,Takashi Shinkawa,Hisashi Koga,Kosei Takeuchi,Toshiaki Isobe,Michihiro Igarashi
概要	Identification of proteins in the mammalian growth cone has the potential to advance our understanding of this critical regulator of neuronal growth and formation of neural circuit; however, to date, only one growth cone marker protein, GAP-43, has been reported. Here, we successfully used a proteomic approach to identify 945 proteins present in developing rat forebrain growth cones, including highly abundant, membrane-associated and actin-associated proteins. Almost 100 of the proteins appear to be highly enriched in the growth cone, as determined by quantitative immunostaining, and for 17 proteins, the results of RNAi suggest a role in axon growth. Most of the proteins we identified have not previously been implicated in axon growth and thus their identification presents a significant step forward, providing marker proteins and candidate neuronal growth-associated proteins.
DOI	10.1073/pnas.0904092106
PMID	19805073