ワタナベ　ケイスケ   渡辺　啓介    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2008/10
形態種別	論文
査読	査読あり
標題	Netrin-1 Acts as a Repulsive Guidance Cue for Sensory Axonal Projections toward the Spinal Cord
執筆形態	共著
掲載誌名	JOURNAL OF NEUROSCIENCE
掲載区分	国外
出版社・発行元	SOC NEUROSCIENCE
巻・号・頁	28(41),pp.10380-10385
著者・共著者	Tomoyuki Masuda,Keisuke Watanabe,Chie Sakuma,Kazuhiro Ikenaka,Katsuhiko Ono,Hiroyuki Yaginuma
概要	During early development, the ventral spinal cord expresses chemorepulsive signals that act on dorsal root ganglion (DRG) axons to help orient them toward the dorsolateral part of the spinal cord. However, the molecular nature of this chemorepulsion is mostly unknown. We report here that netrin-1 acts as an early ventral spinal cord-derived chemorepellent for DRGaxons. In the developing mouse spinal cord, netrin-1 is expressed in the floor plate of the spinal cord, and the netrin receptor Unc5c is expressed in DRGneurons. We show that human embryonic kidney cell aggregates secreting netrin-1 repel DRG axons and that netrin-1-deficient ventral spinal cord explants lose their repulsive influence on DRG axons. In embryonic day 10 netrin-1 mutant mice, we find that DRG axons exhibit transient misorientation. Furthermore, by means of gain-of-function analyses, we show that ectopic netrin-1 in the dorsal and intermediate spinal cord prevents DRGaxons from being directed toward the dorsal spinal cord. Together, these findings suggest that netrin-1 contributes to the formation of the initial trajectories of developing DRG axons as a repulsive guidance cue.
DOI	10.1523/JNEUROSCI.1926-08.2008
ISSN	0270-6474
PMID	18842897