ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	1997/11
形態種別	論文
査読	査読あり
標題	Thermosensing properties of mutant aspartate chemoreceptors with methyl-accepting sites replaced singly or multiply by alanine.
執筆形態	共著
掲載誌名	Journal of bacteriology
掲載区分	国外
巻・号・頁	179(21),pp.6573-80
著者・共著者	◎Nishiyama S, Nara T, Homma M, Imae Y, Kawagishi I
概要	The aspartate chemoreceptor Tar has a thermosensing function that is modulated by covalent modification of its four methylation sites. In this study, we carried out alanine-scanning mutagenesis of the methylation sites. Although alanine substitutions influenced the signaling bias and the methylation level, all of the mutants retained aspartate-sensing function. Those with single substitutions had almost normal thermosensing properties, indicating that substitutions at any particular methylation site do not seriously impair thermosensing function. Warm sensors were found among mutants retaining two glutamate residues, and cold sensors were found among those with one or no glutamate residue. This result suggests that the negative charge at the methylation sites is one factor that determines thermosensor phenotypes. Thus, the different thermosensing phenotypes that result from covalent modification of the methylation sites may reflect distinct structural states.
ISSN	0021-9193
PMID	9352902