ノズミ　モトヒロ   野住　素広    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2017/02/28
形態種別	論文 - 研究論文（学術雑誌）
査読	査読あり
標題	Coordinated Movement of Vesicles and Actin Bundles during Nerve Growth Revealed by Superresolution Microscopy.
執筆形態	その他 - 指定なし
掲載誌名	Cell reports
掲載区分	国外
巻・号・頁	18(9),pp.2203-2216
担当区分	筆頭著者
著者・共著者	Motohiro Nozumi,Fubito Nakatsu,Kaoru Katoh,Michihiro Igarashi
概要	The growth cone is an essential structure for nerve growth. Although its membrane and cytoskeleton are likely to interact coordinately during nerve growth, the mechanisms are unknown due to their close proximity. Here, we used superresolution microscopy to simultaneously observe vesicles and F-actin in growth cones. We identified a novel vesicular generation mechanism that is independent of clathrin and dependent on endophilin-3- and dynamin-1 and that occurs proximal to the leading edge simultaneously with fascin-1-dependent F-actin bundling. In contrast to conventional clathrin-dependent endocytosis, which occurs distal from the leading edge at the basal surfaces of growth cones, this mechanism was distinctly observed at the apical surface using 3D imaging and was involved in mediating axon growth. Reduced endophilin or fascin inhibited this endocytic mechanism. These results suggest that, at the leading edge, vesicles are coordinately generated and transported with actin bundling during nerve growth.
DOI	10.1016/j.celrep.2017.02.008
PMID	28249165