ワタナベ　ケイスケ   渡辺　啓介    所属   新潟薬科大学  医療技術学部 臨床検査学科    職種   教授
言語種別	英語
発行・発表の年月	2011/06
形態種別	論文
査読	査読あり
標題	Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon
執筆形態	共著
掲載誌名	DEVELOPMENT
掲載区分	国外
出版社・発行元	COMPANY OF BIOLOGISTS LTD
巻・号・頁	138(12),pp.2499-2509
著者・共著者	Shengxi Wu,Shigeyuki Esumi,Keisuke Watanabe,Jing Chen,Kouichi C. Nakamura,Kazuhiro Nakamura,Kouhei Kometani,Nagahiro Minato,Yuchio Yanagawa,Kaori Akashi,Kenji Sakimura,Takeshi Kaneko,Nobuaki Tamamaki
概要	In the embryonic neocortex, neuronal precursors are generated in the ventricular zone (VZ) and accumulate in the cortical plate. Recently, the subventricular zone (SVZ) of the embryonic neocortex was recognized as an additional neurogenic site for both principal excitatory neurons and GABAergic inhibitory neurons. To gain insight into the neurogenesis of GABAergic neurons in the SVZ, we investigated the characteristics of intermediate progenitors of GABAergic neurons (IPGNs) in mouse neocortex by immunohistochemistry, immunocytochemistry, single-cell RT-PCR and single-cell array analysis. IPGNs were identified by their expression of some neuronal and cell cycle markers. Moreover, we investigated the origins of the neocortical IPGNs by Cre-loxP fate mapping in transgenic mice and the transduction of part of the telencephalic VZ by Cre-reporter plasmids, and found them in the medial and lateral ganglionic eminence. Therefore, they must migrate tangentially within the telencephalon to reach the neocortex. Cell-lineage analysis by simple-retrovirus transduction revealed that the neocortical IPGNs self-renew and give rise to a small number of neocortical GABAergic neurons and to a large number of granule and periglomerular cells in the olfactory bulb. IPGNs are maintained in the neocortex and may act as progenitors for adult neurogenesis.
DOI	10.1242/dev.063032
ISSN	0950-1991
PMID	21561989