ナカムラ　スミコ   Sumiko Nakamura   中村　澄子    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   特任准教授
言語種別	英語
発行・発表の年月	2020/07
形態種別	論文
査読	査読あり
標題	Development of a novel formula for estimating the amylose content of starch using japonica milled rice flours based on the iodine absorption curve
執筆形態	共著
掲載誌名	Bioscience, Biotechnology, and Biochemistry
掲載区分	国内
出版社・発行元	日本農芸化学会
巻・号・頁	84
著者・共著者	Sumiko Nakamura, Hiroki Yamaguchi, Yuu Benitani, Ken'ichi Ohtsubo
概要	We developed a formula that achieved easy, rapid, and accurate determination of the AACs using milled rice flour instead of rice starch. The apparent amylose content (AAC) can be measured using an iodine colorimetric method to quantitate. Starch preparation generally takes long time and is labor-intensive. We developed a novel formula for estimating the AACs of starch, using milled rice flours from a japonica rice cultivar, according to the iodine light-absorbance curve. The AACs of starch from the rice of 48 japonica species were 1.2–1.3-times higher than those using milled rice flour. We developed a novel formula for estimating the AACs of starch from japonica rice cultivars using milled rice flours according to the iodine absorption curve. The determination coefficients (R2) were 0.80 for calibration and 0.89 for validation, which showed that this equation can be applied to unknown japonica rice samples.