ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2007/08
形態種別	論文
査読	査読あり
標題	Fimbrial proteins of Porphyromonas gingivalis mediate in vivo virulence and exploit TLR2 and complement receptor 3 to persist in macrophages.
執筆形態	共著
掲載誌名	Journal of immunology (Baltimore, Md. : 1950)
掲載区分	国外
巻・号・頁	179(4),pp.2349-58
著者・共著者	◎Wang Min, Shakhatreh Muhamad-Ali K, James Deanna, Liang Shuang, Nishiyama So-ichiro, Yoshimura Fuminobu, Demuth Donald R, Hajishengallis George
概要	Porphyromonas gingivalis is an oral/systemic pathogen implicated in chronic conditions. The virulence of this pathogen partially depends upon expression of fimbriae comprising polymerized fimbrillin (FimA) associated with quantitatively minor proteins (FimCDE). In this study, we show that isogenic mutants lacking FimCDE are dramatically less persistent and virulent in a mouse periodontitis model. Strikingly, native fimbriae allowed P. gingivalis to exploit the TLR2/complement receptor 3 pathway for intracellular entry, inhibition of IL-12p70, and persistence in macrophages. This virulence mechanism also required FimCDE. These results highlight a hitherto unknown mechanism of immune evasion by P. gingivalis that is surprisingly dependent upon minor constituents of its fimbriae, and support the concept that pathogens evolved to manipulate innate immunity for promoting adaptive fitness and thus their capacity to cause disease.
ISSN	0022-1767
PMID	17675496