マエダ　タケヒコ   MAEDA TAKEHIKO   前田　武彦    所属   新潟薬科大学  薬学部 薬学科    職種   教授
言語種別	英語
発行・発表の年月	2025/04
形態種別	論文（従来区分）
査読	査読あり
標題	Semaphorin3A Knockdown Upregulates miR-20b-5p to Suppress Cyclin D1 expression and Cell Proliferation in High Bone-Metastatic Lung Cancer Cell
執筆形態	共著（従来区分）
掲載誌名	BPB Reports
掲載区分	国外
出版社・発行元	硬組織再生生物学会
巻・号・頁	34(2),pp.49-56
著者・共著者	Hasegawa Takuya, Asano Yoshihisa, Maeda Takehiko
概要	<p>Lung cancer remains the leading cause of cancer-related mortality worldwide. In advanced lung cancer stages, bone metastasis is rampant and significantly reduces the quality of life of patients. Members of the semaphorin family, particularly semaphorin 3A (SEMA3A), have been implicated in lung cancer growth and metastasis; however, their role remains unclear. In this study, we investigated the relationship between SEMA3A signaling and microRNA (miRNA) regulation in a highly bone metastatic lung cancer cell line (HARA-B4). Microarray analysis and qRT-PCR revealed upregulation of miR-20b-5p expression in SEMA3A knockdown cells. Target pathway analysis identified cyclin D1(CCND1), a key regulator of cell cycle progression, as the downstream target. miR-20b-5p mimic reduced CCND1 expression and suppressed cell proliferation in HARA-B4. These findings suggest that SEMA3A-dependent tumor growth is mediated by the increased CCND1 expression resulting from reduced miR-20b-5p expression. Understanding the dual roles of SEMA3A and miR-20b-5p may provide potential therapeutic and diagnostic avenues for treating metastatic bone lung cancer.</p>
DOI	10.2485/jhtb.34.49
ISSN	13417649
NAID	1390585407758457856