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ワタナベ ケイスケ
渡辺 啓介 所属 新潟薬科大学 医療技術学部 臨床検査学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/03 |
| 形態種別 | 論文 |
| 査読 | 査読あり |
| 標題 | A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors |
| 執筆形態 | 共著 |
| 掲載誌名 | NATURE NEUROSCIENCE |
| 掲載区分 | 国外 |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 15(3),pp.373-U193 |
| 著者・共著者 | Ryota Shinohara,Dean Thumkeo,Hiroshi Kamijo,Naoko Kaneko,Kazunobu Sawamoto,Keisuke Watanabe,Hirohide Takebayashi,Hiroshi Kiyonari,Toshimasa Ishizaki,Tomoyuki Furuyashiki,Shuh Narumiya |
| 概要 | In brain development, distinct types of migration, radial migration and tangential migration, are shown by excitatory and inhibitory neurons, respectively. Whether these two types of migration operate by similar cellular mechanisms remains unclear. We examined neuronal migration in mice deficient in mDia1 (also known as Diap1) and mDia3 (also known as Diap2), which encode the Rho-regulated actin nucleators mammalian diaphanous homolog 1 (mDia1) and mDia3. mDia deficiency impaired tangential migration of cortical and olfactory inhibitory interneurons, whereas radial migration and consequent layer formation of cortical excitatory neurons were unaffected. mDia-deficient neuroblasts exhibited reduced separation of the centrosome from the nucleus and retarded nuclear translocation. Concomitantly, anterograde F-actin movement and F-actin condensation at the rear, which occur during centrosomal and nuclear movement of wild-type cells, respectively, were impaired in mDia-deficient neuroblasts. Blockade of Rho-associated protein kinase (ROCK), which regulates myosin II, also impaired nuclear translocation. These results suggest that Rho signaling via mDia and ROCK critically regulates nuclear translocation through F-actin dynamics in tangential migration, whereas this mechanism is dispensable in radial migration. |
| DOI | 10.1038/nn.3020 |
| ISSN | 1097-6256 |
| PMID | 22246438 |