ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2014/01
形態種別	論文
査読	査読あり
標題	Mutational analysis of the P1 phosphorylation domain in Escherichia coli CheA, the signaling kinase for chemotaxis.
執筆形態	共著
掲載誌名	Journal of bacteriology
掲載区分	国外
巻・号・頁	196(2),pp.257-64
著者・共著者	◎Nishiyama So-ichiro, Garzón Andrés, Parkinson John S
概要	The histidine autokinase CheA functions as the central processing unit in the Escherichia coli chemotaxis signaling machinery. The CheA phosphorylation site, His-48, lies in the N-terminal P1 domain, which must engage the CheA ATP-binding domain, P4, to initiate an autophosphorylation reaction cycle. The docking determinants for the P1-P4 interaction have not been experimentally identified. One set of P1 mutants identified amino acid replacements at surface-exposed residues distal to His-48. These lesions reduced the rate of P1 transphosphorylation by P4. However, once phosphorylated, the mutant P1 domains transferred phosphate to CheY at the wild-type rate. Thus, these P1 mutants appear to define interaction determinants for P1-P4 docking during the CheA autophosphorylation reaction.
DOI	10.1128/JB.01167-13
ISSN	1098-5530
PMID	24163342