ニシヤマ　ソウイチロウ   Soichiro Nishiyama   西山　宗一郎    所属   新潟薬科大学  応用生命科学部 応用生命科学科    職種   准教授
言語種別	英語
発行・発表の年月	2016/02
形態種別	論文
標題	Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants.
執筆形態	共著
掲載誌名	Scientific reports
掲載区分	国外
巻・号・頁	6,pp.20866
著者・共著者	◎Nishiyama So-ichiro, Takahashi Yohei, Yamamoto Kentaro, Suzuki Daisuke, Itoh Yasuaki, Sumita Kazumasa, Uchida Yumiko, Homma Michio, Imada Katsumi, Kawagishi Ikuro
概要	Vibrio cholerae, the etiological agent of cholera, was found to be attracted by taurine, a major constituent of human bile. Mlp37 was found to mediate taxis to taurine as well as L-serine and other amino acids. Isothermal titration calorimetry demonstrated that a purified periplasmic fragment of Mlp37 binds directly to taurine and L-serine. Crystal structures of the periplamic domain of Mlp37 revealed that L-serine and taurine bind to the membrane-distal PAS domain in essentially in the same way. The structural information was supported by characterising the in vivo properties of alanine-substituted mutant forms of Mlp37. Taken together, we conclude that Mlp37 serves as the major chemoreceptor for taurine and various amino acids.
DOI	10.1038/srep20866
ISSN	2045-2322
PMID	26878914